Pixelgen

About Pixelgen

Pixelgen Technologies specializes in advancing the field of 3D spatial analysis of cell surface proteins, leveraging its proprietary Molecular Pixelation (MPX) technology. This innovative approach is aimed at enhancing the understanding of cell biology, disease mechanisms, and the development of new drugs and diagnostics. By enabling detailed examination of cell surface proteins and their spatial relationships within single cells, Pixelgen's technology provides insights that are unattainable through traditional analytical methods, which often lack the spatial resolution and multiplexing capabilities offered by MPX [1](https://www.pixelgen.com/)[2](https://www.pixelgen.com/products/). The core features of Pixelgen's MPX technology include 3D spatial single-cell proteomics, which facilitates high-resolution mapping of cell surface proteins. This technology supports deep phenotyping, particularly of immune cells, and allows for the simultaneous analysis of numerous proteins due to its high multiplexing capability using DNA-tagged monoclonal antibodies. Additionally, the high-throughput design enables the processing of a vast number of cells efficiently, supported by a simplified workflow that eliminates complex procedures commonly associated with similar technologies. MPX data is compatible with existing next-generation sequencing (NGS) workflows, complemented by the dedicated Pixelator software for data processing and analysis [2](https://www.pixelgen.com/products/)[4](https://www.pixelgen.one/products/index.html). Use cases for Pixelgen's technology include applications in immunology for understanding immune cell responses, cancer research for studying tumor growth and therapy responses, drug discovery for developing new medications with enhanced specificity, and the creation of diagnostic tools with improved accuracy. This breadth of applications underscores the technology's versatility and impact across various biomedical fields [1](https://www.pixelgen.com/)[2](https://www.pixelgen.com/products/). Pixelgen's key differentiators include its unparalleled 3D spatial resolution and high multiplexing capability, providing insights that are beyond the reach of many traditional single-cell technologies. The streamlined workflow further facilitates accessibility for a broad array of researchers, while integration with existing NGS workflows ensures seamless data processing and analysis [2](https://www.pixelgen.com/products/). Technical specifications for the Pixelgen Single Cell Spatial Proteomics Kit include a research focus on immunology, with digital spatial protein correlation NGS output. The input accommodates 500,000 cells, aliquot up to 1,000 cells for library preparation, and it features a protein panel of 84 targets. The data output capacity is up to 1,000 cells per reaction, offering a resolution of <100 nm/~200 nm and a protocol time of approximately two days [2](https://www.pixelgen.com/products/)[4](https://www.pixelgen.one/products/index.html). The technology is further enriched by integration with computational solutions developed in collaboration with BioLizard, which has optimized Pixelgen's computational pipelines, cloud infrastructure, and reporting tools [3](https://lizard.bio/knowledge-hub/software-engineering-molecular-pixelation). Recent developments include a December webinar focusing on the application of Molecular Pixelation in cancer research. This, coupled with ongoing collaboration with BioLizard, signifies Pixelgen's commitment to continuous innovation and refinement of its analytical tools and platforms [1](https://www.pixelgen.com/)[3](https://lizard.bio/knowledge-hub/software-engineering-molecular-pixelation).

Key Features

• 3D spatial single-cell proteomics
• High-resolution mapping of cell surface proteins
• High multiplexing capability with DNA-tagged antibodies
• Streamlined workflow compatible with NGS
• Comprehensive data analysis with Pixelator software
• Enhanced precision for drug discovery
• Applications in immunology, cancer research, and diagnostics
• Optimized computational solutions with BioLizard collaboration
• Unparalleled spatial resolution
• Seamless integration with existing workflows

FAQs